Immunofluorescence staining of A549 cells with CSB-PA007111LA01HU at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Western blot All lanes: DOK5 antibody at 2µg/ml + Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/15000 dilution Predicted band size: 36, 23 kDa Observed band size: 36 kDa
Immunohistochemistry of paraffin-embedded human tonsil tissue using CSB-PA007111LA01HU at dilution of 1:100
Supplier Page from CUSABIO Technology LLC for DOK5 Antibody